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Written in 1886, the story marks the first appearance of Sherlock Holmes and Dr. Watson, who would become two of the most famous characters in popular fiction. The book's title derives from a speech given by Holmes, an amateur detective, to his friend and chronicler Watson on the nature of his work, in which he describes the story's murder investigation as his "study in scarlet" "There's the scarlet thread of murder running through the colourless skein of life, and our duty is to unravel it, and isolate it, and expose every inch of it. The story, and its main characters, attracted little public interest when it first appeared. Only 11 complete copies of the magazine in which the story first appeared, Beeton's Christmas Annual for 1887, are known to exist now and they have considerable value. Although Conan Doyle wrote 56 short stories featuring Holmes, A Study in Scarlet is one of only four full-length novels in the original canon. The novel was followed by The Sign of the Four, published in 1890.